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Based on relationship between phosphors uptake, partitioning, radiation and temperature, a simulation model of greenhouse cucumber phosphorus uptake and partitioning was established. Different experiment data was used to validate the model. The results showed that the coefficients of determination between predicted and measured value based on the 1∶1 line were 0.91,0.74,0.65,0.72,0.65, respectively, and the root mean squared error between the predicted and the measured value was 0.04g/plant, 0.03%, 0.04%, 0.02%, 0.06%, respectively, for phosphorus uptake, root, stem, leaf and fruit phosphorus content. The model could give satisfactory prediction of phosphorus uptake and root, stem, leaf and fruit phosphorus content of greenhouse cucumber in different seasons.