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馬鈴薯渣蛋白抗氧化肽的酶法制備
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of Anti-oxidation Peptide from Potato Pulp
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    摘要:

    以馬鈴薯渣為原料,，采用酶法將薯渣中的蛋白轉化為具有抗氧化活性的多肽,。以酶解液對DPPH自由基清除率為酶解效果評價指標,，從木瓜蛋白酶,、風味蛋白酶,、中性蛋白酶,、堿性蛋白酶,、胰蛋白酶,、胃蛋白酶等6種商業(yè)蛋白酶中篩選出胰蛋白酶為最佳水解用酶,。通過優(yōu)化試驗,，得出薯渣蛋白最佳酶解條件為：底物質(zhì)量濃度4g/（100mL）、加酶量7%,、pH值8.0,、料液溫度50℃,、酶解時間90min。酶解液稀釋20倍后對DPPH自由基的清除率為72%,，酶解液清除

    Abstract:

    DPPH自由基的EC50值為0.155g/（100mL）,。Protease screening was studied to prepare potato antioxidant peptide using the enzymolysis method. With potato dregs as the raw material and the scavenging rate of hydrolysate to the DPPH radical as an index, neutral protease was screened out from the following six kinds of protease: papain, flavor protease, neutral protease, alkaline protease, trypsin and pepsin. The optimum hydrolysis conditions obtained by the experiments were substrate concentration 4 g/(100mL), 7% enzyme-substrate ratio, pH value 8.0, 50°C and 90min reaction time. After being diluted 20 times, the scavenging rate of hydrolysate liquid to the DPPH radical reached 72%. The EC50 value of hydrolysate scavenging the DPPH radical was 0.155 g/(100mL).

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王蓓,馬海樂,喬瑋.馬鈴薯渣蛋白抗氧化肽的酶法制備[J].農(nóng)業(yè)機械學報,2010,41(Z1):198-202. of Anti-oxidation Peptide from Potato Pulp[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(Z1):198-202.

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