ass日本风韵熟妇pics男人扒开女人屁屁桶到爽|扒开胸露出奶头亲吻视频|邻居少妇的诱惑|人人妻在线播放|日日摸夜夜摸狠狠摸婷婷|制服 丝袜 人妻|激情熟妇中文字幕|看黄色欧美特一级|日本av人妻系列|高潮对白av,丰满岳妇乱熟妇之荡,日本丰满熟妇乱又伦,日韩欧美一区二区三区在线

纖維素分解菌產(chǎn)酶性能優(yōu)化試驗
CSTR:
作者:
作者單位:

作者簡介:

通訊作者:

中圖分類號:

基金項目:


Study on Isolation of Cellulose-decomposing Microorganisms and Its Cellulase-producing Conditions
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 圖/表
  • |
  • 訪問統(tǒng)計
  • |
  • 參考文獻
  • |
  • 相似文獻
  • |
  • 引證文獻
  • |
  • 資源附件
  • |
  • 文章評論
    摘要:

    以甜高粱秸稈為原料,,結(jié)合微生物生態(tài)學(xué)的理論,,從自然環(huán)境中分離篩選出具有高酶活力的纖維素分解菌,。通過剛果紅培養(yǎng)基篩選以及液體培養(yǎng)基的復(fù)篩得到一株供試菌株,選取酶解溫度、起始pH值和接種量3因素,,采用五水平正交旋轉(zhuǎn)回歸組合試驗設(shè)計,進行了產(chǎn)粗酶能力的優(yōu)化試驗。利用SPSS 130的回歸分析方法以及Matlab 7.0的響應(yīng)面分析法,,建立并分析了3個因子對還原糖得率影響的數(shù)學(xué)方程。結(jié)果表明,,所得回歸方程顯著,,擬合情況良好;3個因子對還原糖得率的影響大小依次為:酶解溫度,、起始pH值和接種量,;適宜的酶解條件為:溫度26.64℃,起始pH值4.96,,接種量5%,,此時還原糖含量達到

    Abstract:

    54.19mg/g。. According to the theory of microbial ecology, with sweet sorghum straw as the raw material, cellulose-decomposing fungi, which had high enzyme activity, were isolated from their natural habitat and screened. Meanwhile, the production conditions of cellulose enzyme were optimized based on the factors of temperature, initial pH value, and inoculation quantity, and the influence of these factors on enzyme activity was discussed. Taking Z15 as a test strain, optimization of production capacity of crude enzyme test was carried out, according to three factors, five-level orthogonal rotational regression test design, to investigate the effects on fermentation temperature, initial pH value and inoculation quantity. The yield of reducing sugar was taken as the indicators, and the impact on the target size about the three factors was analyzed. By calculating the analysis, the following results of the impact on the target was obtained: temperature was the first one, inoculation quantity was the last one and initial pH value was the middle. The optimum conditions for enzymatic hydrolysis were obtained: optimum temperature was 26.64℃, optimum initial pH value was 4.96, and optimum inoculation quantity was 5%, at this time reducing sugar content was 54.19mg/g. 

    參考文獻
    相似文獻
    引證文獻
引用本文

孫清,易維明,谷士艷,張春梅,葛竟峰,劉慶玉.纖維素分解菌產(chǎn)酶性能優(yōu)化試驗[J].農(nóng)業(yè)機械學(xué)報,2010,41(Z1):141-144.Study on Isolation of Cellulose-decomposing Microorganisms and Its Cellulase-producing Conditions[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(Z1):141-144.

復(fù)制
分享
文章指標(biāo)
  • 點擊次數(shù):
  • 下載次數(shù):
  • HTML閱讀次數(shù):
  • 引用次數(shù):
歷史
  • 收稿日期:
  • 最后修改日期:
  • 錄用日期:
  • 在線發(fā)布日期:
  • 出版日期:
文章二維碼