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纖維素酶協(xié)同超聲波輔助提取苦瓜多糖工藝優(yōu)化
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Optimization on Cellulase and Ultrasonic Wave Assisted Extraction Technology of Momordica charantia Polysaccharides
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    摘要:

    為了得到纖維素酶協(xié)同超聲波法提取苦瓜多糖的最佳工藝條件,利用Box-Behnken的中心組合設(shè)計(jì)及響應(yīng)面法(RSM)探討了超聲波功率、酶量、料液比、時(shí)間、pH值和溫度等因素的優(yōu)化組合,通過建立二次回歸模型,確定其最佳提取工藝條件為: 超聲波功率390W、纖維素酶量3500U/ g(酶活200U/mg以上),、料液比1∶38、時(shí)間40min,、pH值5,、溫度56℃。在此工藝條件下,,苦瓜多糖的提取率為211%,,比熱水浸提法、超聲波法,、纖維素酶法分別提高了7.8%,、13.5%、7.7%,。結(jié)果表明纖維素酶協(xié)同超聲波法是提高苦瓜多糖得率的有效途徑之一,。

    Abstract:

    To establish the extraction technology of cellulase assisted by ultrasonic wave of Momordica charantia polysaccharides (MCP), the optimization was applied to discuss some factors including ultrasound power, enzyme amount, the ratio of solid to liquid, extracting time, pH value, temperature with BoxBehnken centerunited design and the method of response surface analysis (RSM). Through establishing the second regression model, the optimal parameters were conformed as follows: ultrasound power 390W, cellulase amount 3500U/g (enzyme activity was more than 200U/mg), ratio of solid to liquid 1∶38, extracting time 40min, pH value 5, temperature 56℃. Then the yield of crude Momordica charantia polysaccharides is 21.1%, higher 7.8%, 13.5% and 7.7%, respectively, comparing with the specific heat of flooding, ultrasonic wave and cellulase. These results suggested that cellulase assisted by ultrasonic wave extraction technology was one of the effective ways to improve the yield of Momordica charantia polysaccharides. 

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張利芳,張名位,黃文, 張雁,張瑞芬,魏振承.纖維素酶協(xié)同超聲波輔助提取苦瓜多糖工藝優(yōu)化[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2010,41(11):142-147. Optimization on Cellulase and Ultrasonic Wave Assisted Extraction Technology of Momordica charantia Polysaccharides[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(11):142-147.

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