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輻照對鮮食核桃發(fā)芽率與胚芽內(nèi)源激素含量的影
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of Irradiation on Budding Inhibition and Endogenous Hormones Content of Embryo of Fresh Walnut
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    摘要:

    為探討輻照對鮮核桃的抑芽效果及胚芽內(nèi)源激素與其萌發(fā)的關(guān)系,以遼河4號鮮核桃為試材,用不同劑量60Coγ射線處理后,研究了常溫(20±3)℃下和(0±1)℃冷藏30d后再于（27±1）℃溫度下貯藏核桃的發(fā)芽率及0、0.1,、1.0kGy劑量處理核桃胚芽中內(nèi)源激素含量的變化規(guī)律,。結(jié)果顯示,兩種貯藏條件下,對照樣品胚芽分別在20d和12d萌發(fā),萌發(fā)時內(nèi)源激素ABA（脫落酸）含量下降,GA3（赤霉素）,、IAA（生長素）,、ZR（玉米素核苷）含量及w(GA3)/w(ABA),、w(IAA)/w(ABA)和w(ZR)/w(ABA)的比值增加,以對照樣品減幅和增幅最大,。表明內(nèi)源激素ABA對鮮核桃胚芽萌發(fā)起抑制作用,GA3,、IAA和ZR起促進作用; 0.05kGy劑量輻照處理即可完全抑制鮮核桃胚芽的萌發(fā)。

    Abstract:

    In order to investigate the effect of irradiation on the sprout inhibition and the relationship between endogenous hormones and germination of embryo for fresh walnut, the germination rate and changes of endogenous hormones of embryo for ‘Liaohe4’ fresh walnut were studied, which were exposed to 60Coγ-radiation at different dose and stored at the room temperature (20±3)℃ and at the constant temperature (27±1)℃ after being stored at (0±1)℃ for 30d. The results showed, at the two storage conditions, the control sprouts at the 20d and 12d respectively, accompanied by ABA content decreased, by the increase of the content of GA3, IAA, ZR and the ratio of w(GA3)/w(ABA), w(IAA)/w(ABA) and w(ZR)/w(ABA), with the largest decreasing and increasing rate against control group. It is proved that endogenous hormone ABA is an inhibitory substance, and GA3, IAA, ZR are simulative material during the process of germination, the germination of embryo of fresh walnut is completely inhibited with the radiation dose of 0.05kGy.

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馬艷萍,劉興華,李英超,李小東.輻照對鮮食核桃發(fā)芽率與胚芽內(nèi)源激素含量的影[J].農(nóng)業(yè)機械學(xué)報,2010,41(4):114-118. of Irradiation on Budding Inhibition and Endogenous Hormones Content of Embryo of Fresh Walnut[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(4):114-118.

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