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乳鏈菌肽發(fā)酵玉米漿—糖蜜培養(yǎng)基的優(yōu)化制備
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Optimization Corn Steep Liquor—Molasses Fermentation Medium for Nisin
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    摘要:

    采用單因素試驗(yàn)方法對(duì)影響乳鏈菌肽效價(jià)的培養(yǎng)基組分進(jìn)行了考察。結(jié)果表明，影響乳鏈菌肽效價(jià)的主要因素為糖蜜、玉米漿、K2HPO4、MgSO4。在此基礎(chǔ)上，采用Box-Behnken設(shè)計(jì)及響應(yīng)面分析法確定主要影響因子的最佳質(zhì)量濃度。結(jié)果表明，當(dāng)玉米漿質(zhì)量濃度為233g/L，糖蜜質(zhì)量濃度為27g/L，K2HPO4質(zhì)量濃度為5.0g/L，MgSO4質(zhì)量濃度為0.1g/L時(shí)，乳鏈菌肽效價(jià)為2491.8IU/mL，與預(yù)測(cè)值2511.

    Abstract:

    1IU/mL基本一致。This work reviewed fermentation medium culture of nisin by single factor experiment. The results indicate that nisin activity was mainly influenced of molasses, corn steep liquor, K2HPO4 and MgSO4. Box-Behnken design and response surface analysis methods were applied to confirm optimum concentration of mainly effect factor. The results show that the optimum medium culture was corn steep liquor 233g/L, molasses 27g/L, K2HPO4 5g/L, MgSO4 0.1g/L. Under those conditions, nisin activity is 2491.8IU/mL, which is in consistent with predicted value 2511.1IU/mL. 

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逯家輝,孫瑩,姜麗艷,孟慶繁,李揚(yáng),滕利榮.乳鏈菌肽發(fā)酵玉米漿—糖蜜培養(yǎng)基的優(yōu)化制備[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2009,40(7):158-162. Optimization Corn Steep Liquor—Molasses Fermentation Medium for Nisin[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(7):158-162.

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