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To find reasonable position on the kiwifruit to collect near-infrared spectrum information for quality evaluation, near-infrared spectrum was collected at different locations near the equator on the kiwifruit applying the near-infrared spectrum diffuse reflection technology. Difference analysis on the average absorbance and the noise of the spectrum on the tested locations was carried out. The single factor variance analysis was conducted to the nearinfrared spectrum wave ridge position and the absorbance at different locations, as well as the prediction precision of the Brix quantitative analysis model. The results showed that the variety for the average absorbance and noise for the spectrum at different locations around the equator on the same kiwifruit was low, and the wave ridge position difference was not remarkable. The difference of absorbance was not remarkable at different locations in the same wave ridge position for the same kiwifruit. The relatively standard deviation of the absorbance was greater when the wavelength was longer in the near infrared area. However, the degree of scatter of data was not high at all wavelength at different locations in the same wave ridge position. The deference for the Brix prediction models built by spectrum data at different locations was not remarkable. It is concluded that the near-infrared spectrum information collected at different locations around the equator of the kiwifruit can be used for quality evaluation for the entire fruit without high difference.