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145 fishmeal samples were collected and the calibration models to predict total 17 types of amino acids and total amino acids in fishmeal, such as aspartic acid, methionine, lysine, threonine and so on, were developed by using partial least squares (PLS) technique. The coefficients of determination in calibration set (R2) of chemical analysis values and NIRS prediction values of aspartic acid, methionine, lysine, threonine, glutamic acid, glycine, alanine, valine, isoleucine, leucine, phenylalanine, arginine, proline and total amino acids were all over 0.87, the RSD all less than 10%, RPD all over 3; the R2 of tyrosine was 0.867 8, RSD 8.65%, RPD 2.77; the R2 for histidine, serine and cysteine were 0.900 5, 0.743 6 and 0.354 1, respectively, RSD 14.19%, 17.85% and 33.85%, respectively, RPD 2.96, 1.98 and 1.04, respectively. The results show that NIRS analysis technique can be used to measure the contents of 14 kinds of amino acids, such as aspartic acid, methionine, lysine, threonine and total amino acid in fish meal accurately and to predict tyrosine content roughly. However, it can hardly be employed to measure histidine, serine and cysteine content in practice.